Método de descelularização de pele de tilápia (Oreochromis sp.)

Abstract

Burns are a major aggression in the body that impair the maintenance of the functional characteristics of the skin and the treatment, in the public network, is performed by using the topical antimicrobial silver sulfadiazine 1% until complete repair of the lesion. Recently, biological tissues of animal origin such as pig skin, bovine pericardium and frog skin are commonly used as biological dressings in burn injuries. In this context, aiming at the development of treatments that overcome financial limitations and thinking about more effective treatments, the study seeks to evaluate the efficiency of decellularization processesusing SDS 1% and Triton X-100 and to determine the integrity of the tissue by collagen dosage. The efficiency of decellularization of tilapia skin was evaluated by DNA extraction followed by quantification and electrophoresis in agarose gel and nuclear labeling with 4',6-diamino-2-phenylindol (DAPI). The matrix, after decellularization, was evaluated by collagen dosage. Thus, the process of tissue decellularization achieved 95.1% of protocol 1 and 84.29% of protocol 2 reduction in the amount of DNA in the samples. Cell nuclei were not visible in the DAPI staining, and the absence of DNA bands was observed in the agarose gel. The collagen content did not show major statistical changes when compared to fresh tissue with decellularization. However it was observed that SDS 1% was more effective in decellularization compared to Triton X-100. Taken together, these previous results indicate that the protocol developed using only 1% SDS, presents itself as a means for the decellularization of tilapia skin, with potential application in the regeneration of skin affected by burns.