Os diferentes suplementos de meios de cultura para obtenção de celulas-tronco da polpa dentária podem impactar na taxa de proliferação? Uma revisão sistemática

Abstract

Objective: The cultivation of stem cells is becoming increasingly important, mainly due to the increased use in several areas for regeneration and multiple cell differentiations. This study aims to observe the culture media and supplements evaluating their influence on proliferation rates. Methods: The search was conducted through PubMed, Embase, Scielo, Lilacs, Scopus and Web of Science databases using the keywords “mesenchymal stem cells”, “dental pulp stem cells”, “stem cells from exfoliated deciduous teeth", "culture medium" and "cell proliferation". Included articles were eligible by inclusion criteria. Results: A total of 521 articles were selected, excluding duplicates, of which, after applying the inclusion and exclusion criteria, only 7 were satisfactory for this systematic review. The analysis of supplements was performed after grouping the articles for better comparison. Ascorbic acid, glutamine, glutamax and amphotericin B showed great influence on proliferation rates. Conclusion: The influence of culture media supplements on the cell proliferation rates of DPSCs is undeniable, but in order to advance in the use of stem cells for tissue engineering in dentistry and medicine, further studies will be needed to achieve a standardization of the methodology and the results.