Exercício resistido de alta intensidade e suplementação de produto natural atenuam biomarcadores de estresse oxidativo e danos musculares

Abstract

High-intensity physical exercises can cause oxidative stress if they are not properly neutralized by endogenous and/or exogenous antioxidant agents, in the same way muscle damage if they do not have adequate recovery, which can injure the tissues involved in muscular action. Several medicinal plants have been used as antioxidants and anti-inflammatory drugs. Objective: The present study aimed to evaluate high-intensity resistance physical exercise (EFAI) associated with ethanolic extract (EE) of Schinus Terebentifholius leaves on oxidative parameters and muscle damage in Wistar rats. Methodology: The animals were divided into 04 groups (n=10/group): 1. Control (CG) - animals that did not perform the EFAI session and were treated with vehicle (orally distilled water); 2. Acute exercise (AS) - subjected to an acute exercise session; 3. Exercise + vehicle (EV) - composed of animals that underwent the session and were treated with vehicle and 4. Exercise + extract (EX) animals administered with EE from Schinus Terebenthifolius (100mg/Kg, orally) and that underwent the treatment session exercise. EE from Schinus Terebenthifolius demonstrated, in vitro, high antioxidant activity (13.88 ± 0.36 μg/mL). Before the experimental period, lactate was measured in the pre and post moments of the EA (p<0.0001) and EX (p<0.0001) groups. After the acute session, the following were evaluated: oxidative stress {malondialdehyde (MDA), sulfhydryl groups (SH) and ferric reduced antioxidant (FRAP)}, muscle damage (creatine kinase (CK) and lactate dehydrogenase (LDH)), alanine aminotransferase (ALT ) and aspartate aminotransferase (AST). Results: In in vivo analyses, there were significant differences in the EX group compared to EA and EV, respectively: hepatic (MDA: p<0.0001 and SH: p=0.0033, in both; FRAP: p=0.0011 and p= 0.0047), muscle (MDA, SH and FRAP: p<0.0001, in both; CK: p=0.0001 and p<0.0001; LDH: p<0.0001, in both), serum levels (MDA: p=0.0003 and p=0.0012; SH: p=0.0056 and p=0.0200; FRAP: p=0.0017 and p=0.0165). There was no significant difference in ALT and AST markers. Conclusion: It is concluded that the EE of Schinus Terebenthifolius associated with EFAI attenuated oxidative and muscle damage in rats.