Aproveitamento do permeado de soro obtido por osmose reversa como meio de cultivo para produção de biomolécula por Pseudomonas spp

Abstract

Whey permeate is a unique by-product that contains organic molecules with high polluting potential when not treated properly. It originates from the membrane separation process that segregates the protein part of whey, called retentate. Secondarily, permeate is generated, a by-product of low commercial value. The objective of the study was to evaluate the growth of different strains of Pseudomonas spp. in whey permeate obtained by reverse osmosis (RO), supplemented or not with mineral salts, as well as to determine the profile of metabolites produced over the incubation period in order to obtain compounds of biotechnological interest. Four strains of Pseudomonas spp. were used: Pseudomonas aeruginosa LBI (Industrial Biotechnology Laboratory); Pseudomonas putida CCMI 852 (Industrial Microorganism Culture Collection) and two microorganisms isolated from a whey concentration plant called (IBPCS) IBPCS 09 and IBPCS 14, presumptively identified as belonging to the genus Pseudomonas spp. The bacteria were tested for their ability to grow in whey permeate obtained by OR, followed by qualitative and quantitative verification of metabolite formation. Cell growth was evaluated by optical density in the 600 nm range (OD 600 nm) using a spectrophotometer. Spectrophotometric readings showed the development of cell mass in the permeate cultures after 72 hours. The initial OD 600 nm of 0.8 contained in the medium was increased to 1.288, 1.305, 2.210, and 2.454, respectively, by the microorganisms IBPCS 09, IBPCS14, Pseudomonas putida CCMI 852, and Pseudomonas aeruginosa LBI. The evaluation of the potential for obtaining metabolites of biotechnological interest showed concentrations of 0.621 mg.mL-1 of citric acid, 12.151 mg.mL-1 of isocitric acid, 0.246 mg.mL-1 of formic acid, and 0.558 mg. mL-1 of lactulose in the supernatant samples resulting from the culture. The serum permeate obtained by reverse osmosis proved to be an efficient culture medium for the growth of the microorganisms used in this study, in addition to showing potential for application in biotechnological processes.