Caracterização morfométrica dos estádios de Haemoproteus columbae Kruse, 1890 (protista, apicomplexa) no hospedeiro vertebrado Columba livia Gmelikm, 1879 e no hospedeiro invertebrado Pseudolynchia canarienses (Macquart, 1839) (Díptera: hippoboscidae) no município de Juiz de Fora, MG

Abstract

Haemoproteus columbae Kruse, 1890, is a wide geographically distributed hemoparasite. It has as vertebrate intermediary hosts, Columba livia (Gmelim, 1879), and as invertebrate definitive hosts, flies of the Hippoboscidae family, Pseudolynchia canarienses Macquart (1839). The aim of the present study was to evaluate the prevalence and the parasitemia of H. columbae in pigeons, and to morphologically and morphometrically characterize this hemoparasite’s different stadiums (macrogametocytes, microgametocytes, ookinetes, macrogamets, microgamets, oocysts, sporozoites). Amongst the 41 pigeons captured in Juiz de Fora city, MG state, it was found a 95% of prevalence, being 83.3% found in the 35 animals captured in the Mariano Procópio Museum (Locality 1) and 100% in the six animals captured in the Jarbas de Lery Santos Square (Locality 2). Parasitemia was evaluated by making a counting of parasited red blood cells, in 100 microscopical fields of the pigeons’ blood smears, with a 1000x increase. The differential quantification of the eritrocitic forms registered the following average numbers, with respective standard deviations and percentile relations: macrogametocytes 448.44±1492.25; 59.8%; microgametocytes 205.73±547.11; 27.1%; young forms 36±102.31; 4.8%; poliparasitism 58.78±286,91; 7.8%. In localities 1 and 2, it were registered, respectively, the following average numbers: macrogametocytes 519.77±1607.46 and 29.5±21.28; microgametocytes 237.9±587.41 and 20.83±24.46; young forms 42.31±109.76 and 3±3.35; poliparasitism 68.86±310.05 and zero. The length and width averages of the eritrocitic forms found, in localities 1 and 2, respectively, were the following ones: macrogametocytes 12.15mm±1.88 x 5.2mm±1.89 and 11.86mm±1.63 x 4.65mm±1.82; microgametocytes 11.61mm±1.61 x 3.49±1.23mm and 11.49mm±1.25 x 3.33mm±0.89. Length and width averages of H. columbae mensurated stadiums in P. canarienses, collected on hosts captured in locality 1 were, respectively, the following ones: macrogamets 7.44x6.33mm; ookinetes 15.92±3.5x3.38mm±0.87; sporozoites 9.13±2.67x1.47mm±0.52.