Avaliação da atividade anti-inflamatória de aminoálcoois lipofílicos

Abstract

The inflammatory process is a coordinated response to infections and tissue damage by activating the immune response and leading to the release of pro-inflammatory substances, such as nitric oxide, TNF-a and IL-1β. In this study a series of lipophilic long chain aliphatic aminoalcohols was evaluated in RAW 264.7 macrophage lineage and inflamed peritoneal macrophages of mice BALB/c, to verify cell viability by the MTT method, the production of nitric oxide by Griess method, as well as dosage TNF-a and IL-1β by ELISA. Lipophilic amino alcohols that presented good results in vitro, were used in vivo treatment of mice in models of the carrageenan- induced paw edema in mouse and measure the levels of TNF-a and IL-1β in the supernatant of macerated of footpad mice as well as in the delayed hypersensitivity reaction (RHT) to ovalbumin model. Cultures of RAW 264.7 macrophage lineage with aminoalcohols in the presence of different concentrations (1, 0.5, 0.05 and 0.005 mg / mL) were evaluated. The aminoalcohols tested did not affect cell viability and inhibit the production of NO and TNF-a, most of the concentrations tested, but without altering the production of IL-1β. In cultures of primary macrophages the amino alcohols 4a and 4b, which altered cell viability and did not induce a decrease in NO production and pro-inflammatory cytokines TNF-a and IL-1β were tested. The amino alcohols 4a and 4b were chosen for testing in vivo. Dexamethasone is chosen as the reference drug for treating various inflammatory diseases, but have unwanted side effects due to the complete suppression of immune response. The amino alcohol 4b showed reduced edema induced in mouse paw by carrageenan, and decreased TNF-a and IL-1β as effective as treatment with dexamethasone. In the anti-ovalbumin delayed hypersensitivity reaction the aminoalcohols 4a and 4b showed reduction in paw edema mice similar dexamethasone. In function of the results observed in vitro and in vivo compound 4b showed promissory anti-inflammatory properties.